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Difference between SOC and NZY+ Amine broth

Difference between SOC and NZY+ Amine broth - Protocols and Methods Forum

Difference between SOC and NZY+ Amine broth - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 12-29-2004, 03:28 PM
R Breton
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Default Difference between SOC and NZY+ Amine broth



Hi there,
Im using the Quikchange kit from stratagene for the first and in the
procedure it says to use NZY amine broth, whilst the procedure for the
XL1 Blue Supercompetant cells says to use SOC broth. The only
difference i can see is that SOC contains 20g/l tryptone whilst NZY
contains 10g/l of NZ Amine mix. What is the difference between these
two sources, as far as i can see they're both caesin digests.

Many thanks and a happy new year!
Robert Breton
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  #2  
Old 03-03-2005, 10:12 PM
CAS
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Default Difference between SOC and NZY+ Amine broth


R Breton wrote:
the

To belatedly add my two cents to the mix: I typically just use LB with
the Quickchange protocol. Works fine, and I always have some on hand.


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  #3  
Old 03-04-2005, 12:58 PM
D.K.
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Default Difference between SOC and NZY+ Amine broth

In article <1109887921.300977.119450@f14g2000cwb.googlegroups .com>, "CAS" <[Only registered users see links. ]> wrote:

That, and any generic home-made electrocompetent cells are always
better than Stratagene's chemical ones anyway.

DK

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