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| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
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#1
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| Hi there, Im using the Quikchange kit from stratagene for the first and in the procedure it says to use NZY amine broth, whilst the procedure for the XL1 Blue Supercompetant cells says to use SOC broth. The only difference i can see is that SOC contains 20g/l tryptone whilst NZY contains 10g/l of NZ Amine mix. What is the difference between these two sources, as far as i can see they're both caesin digests. Many thanks and a happy new year! Robert Breton |
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#2
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| R Breton wrote: the To belatedly add my two cents to the mix: I typically just use LB with the Quickchange protocol. Works fine, and I always have some on hand. |
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#3
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| amine , broth , difference , nzy , soc |
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