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Q: multiple bands on Quikchange

Q: multiple bands on Quikchange - Protocols and Methods Forum

Q: multiple bands on Quikchange - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 12-08-2004, 01:48 PM
Woo Cheol Lee
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Default Q: multiple bands on Quikchange



Dear molbio gurus,

I used PfuTurbo and PfuUltra for quikchange mutagenesis and got
different results. I have either a small band or a (correct)
large band with PfuUltra while PfuTurbo yields two bands with
similar sizes (probably larger one being the correct one?).

I uploaded the photo of my quikchange results.

[Only registered users see links. ]

What is the identity of those smaller bands?

Thanks in advance,

Woo
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  #2  
Old 12-08-2004, 05:11 PM
Duncan Clark
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Default Q: multiple bands on Quikchange

Historians believe that in newspost
<[Only registered users see links. ] > on Wed, 8 Dec 2004,
Woo Cheol Lee <[Only registered users see links. ]> penned the following literary
masterpiece:

Mis-priming by one or more primers?

You appear to have an awful lot of dimers or primers in the reaction
looking at the very small band in the Turbo & Ultra2 photos?

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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