Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

FW: Purification of Genomic DNA

FW: Purification of Genomic DNA - Protocols and Methods Forum

FW: Purification of Genomic DNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 11-09-2004, 04:08 PM
Deanne Bell
Posts: n/a
Default FW: Purification of Genomic DNA

>Subject: Purification of Genomic DNA
Hi Sam
It might take a couple of correspondences in order to help you. I would
ask 2 questions: 1) what type of tissue/organism are you trying to get
DNA from? And 2) what do you want to do with the DNA / what is your
downstream procedure?

Here's some input that might help form your next questions:
Most protocols for isolation of DNA mean 'the isolation of all DNA' in
the cell. The predominant DNA found in cells is the DNA that makes up
chromosomes (aka genomic DNA).

There are basically a handful of different techniques to isolate DNA.
In my work, I have found that in order to get good, high quality,
genomic DNA, it is necessary to test a variety of isolation procedures
right from the start. For the most part, each of the different
organisms or species I study requires a different protocol - I work with
plants, insects and bacteria. I have not found the perfect
applies-to-all type of protocol. I would be happy to suggest protocols
for you to try, but I am not sure which protocol to send.

Another question: how do you know that the 'traditional method of my
lab' didn't work? What methods did you use to visualize or quantify and
assess your DNA isolation?

Hope this helps
Deanne Bell
USDA Agricultural Research Service
Crop Diseases, Pests & Genetics
9611 South Riverbend Avenue
Parlier, CA 93648
voice (559) 596-2806
fax (559) 596-2921
[Only registered users see links. ]
Reply With Quote
Old 11-09-2004, 05:12 PM
Posts: n/a
Default Purification of Genomic DNA


to answer your questions,
1. my genomic dna was extracted from heart tissue of rats
2. i would like to do pcr reactions for the downstream procedure

how come i know the traditional procedure didnt work? well...i measured the
OD260 and OD280 and the genomic dna purified is out of the normal range..i
mean the ratio of OD260 and OD280 and the pellet precipitated during the
process was extremely large and it shouldnt be like that...(From the
experience of my labmates) Thanks


Reply With Quote

dna , genomic , purification

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Genomic DNA purification ramvenkat1983 DNA Forum 0 03-18-2010 10:19 AM
genomic DNA PCR: Troubleshooting/General Questions ChameleonAmy PCR - Polymerase Chain Reaction Forum 3 01-12-2010 03:55 PM
inactive enzyme after purification with Ni-TED piefke Recombinant Protein Forum 0 05-02-2008 08:14 AM
Purification of Genomic DNA Samantha Protocols and Methods Forum 1 11-10-2004 08:41 AM
FW: Purification of Genomic DNA Deanne Bell Protocols and Methods Forum 2 11-09-2004 08:40 PM

All times are GMT. The time now is 11:50 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11992 seconds with 16 queries