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FW: Purification of Genomic DNA

FW: Purification of Genomic DNA - Protocols and Methods Forum

FW: Purification of Genomic DNA - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-09-2004, 04:08 PM
Deanne Bell
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Default FW: Purification of Genomic DNA



>Subject: Purification of Genomic DNA
~*~*~*~*~*~*~*~*~*~
Hi Sam
It might take a couple of correspondences in order to help you. I would
ask 2 questions: 1) what type of tissue/organism are you trying to get
DNA from? And 2) what do you want to do with the DNA / what is your
downstream procedure?

Here's some input that might help form your next questions:
Most protocols for isolation of DNA mean 'the isolation of all DNA' in
the cell. The predominant DNA found in cells is the DNA that makes up
chromosomes (aka genomic DNA).

There are basically a handful of different techniques to isolate DNA.
In my work, I have found that in order to get good, high quality,
genomic DNA, it is necessary to test a variety of isolation procedures
right from the start. For the most part, each of the different
organisms or species I study requires a different protocol - I work with
plants, insects and bacteria. I have not found the perfect
applies-to-all type of protocol. I would be happy to suggest protocols
for you to try, but I am not sure which protocol to send.

Another question: how do you know that the 'traditional method of my
lab' didn't work? What methods did you use to visualize or quantify and
assess your DNA isolation?

Hope this helps
Deanne Bell
USDA Agricultural Research Service
Crop Diseases, Pests & Genetics
9611 South Riverbend Avenue
Parlier, CA 93648
voice (559) 596-2806
fax (559) 596-2921
[Only registered users see links. ]
---
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  #2  
Old 11-09-2004, 05:12 PM
Samantha
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Default Purification of Genomic DNA

hi

to answer your questions,
1. my genomic dna was extracted from heart tissue of rats
2. i would like to do pcr reactions for the downstream procedure

how come i know the traditional procedure didnt work? well...i measured the
OD260 and OD280 and the genomic dna purified is out of the normal range..i
mean the ratio of OD260 and OD280 and the pellet precipitated during the
process was extremely large and it shouldnt be like that...(From the
experience of my labmates) Thanks

Sam


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