Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Nusieve agarose gel

Nusieve agarose gel - Protocols and Methods Forum

Nusieve agarose gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-31-2004, 03:54 PM
Samantha
Guest
 
Posts: n/a
Default Nusieve agarose gel



hi everyone

whats the difference between nusieve agarose gel and normal agarose gel for
DNA electrophoresis? if the protocol states to use 2% nusieve agarose gel,
then can i use 2% agarose gel instead or adjust the percentage a bit for
normal agarose gel to replace nusieve agarose? (as my lab doesnt have
nusieve agarose?)
thanks

samantha


Reply With Quote
  #2  
Old 11-05-2004, 10:32 AM
Rafael Maldonado
Guest
 
Posts: n/a
Default Nusieve agarose gel

Samantha wrote:
Nusieve is (was, at leas) a trademark of FMC; which probably have merged
in a bigger biotech company by now. It is special agarose for very small
DNA fragments. If you do not have it, you may use 3-4% "normal" agarose,
but it will not have the same resolution than Nusieve. Be careful, with
that agarose concentration I would not recommend microwave, but ina
boiling water bath.

--
Rafael Maldonado, Ph. D.
Division of Genetics
University of Alicante
Spain
Reply With Quote
Reply

Tags
agarose , gel , nusieve


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agarose gel electrophoresis of PCR products - Method and Protocol domba PCR - Polymerase Chain Reaction Forum 12 02-24-2012 03:39 AM
agarose gel purification of cloning vector mwcrepeau@hawaii.rr.com Protocols and Methods Forum 5 06-17-2010 09:33 AM
vertical agarose gel ilayda sen Protocols and Methods Forum 0 08-01-2006 03:42 AM
Agarose Gel Electrophoresis and DNA Fragment Purification domba Molecular Biology Articles and Protocols 0 07-31-2006 11:17 PM
FW: Nusieve agarose gel Deanne Bell Protocols and Methods Forum 0 11-05-2004 03:15 PM


All times are GMT. The time now is 08:38 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11431 seconds with 16 queries