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#1
10-31-2004, 02:54 PM
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 Nusieve agarose gel hi everyone whats the difference between nusieve agarose gel and normal agarose gel for DNA electrophoresis? if the protocol states to use 2% nusieve agarose gel, then can i use 2% agarose gel instead or adjust the percentage a bit for normal agarose gel to replace nusieve agarose? (as my lab doesnt have nusieve agarose?) thanks samantha  |
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#2
11-05-2004, 09:32 AM
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| Nusieve agarose gel Samantha wrote: Nusieve is (was, at leas) a trademark of FMC; which probably have merged in a bigger biotech company by now. It is special agarose for very small DNA fragments. If you do not have it, you may use 3-4% "normal" agarose, but it will not have the same resolution than Nusieve. Be careful, with that agarose concentration I would not recommend microwave, but ina boiling water bath. -- Rafael Maldonado, Ph. D. Division of Genetics University of Alicante Spain |
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| agarose , gel , nusieve |
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