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Nusieve agarose gel

Nusieve agarose gel - Protocols and Methods Forum

Nusieve agarose gel - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

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Old 10-31-2004, 03:54 PM
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Default Nusieve agarose gel

hi everyone

whats the difference between nusieve agarose gel and normal agarose gel for
DNA electrophoresis? if the protocol states to use 2% nusieve agarose gel,
then can i use 2% agarose gel instead or adjust the percentage a bit for
normal agarose gel to replace nusieve agarose? (as my lab doesnt have
nusieve agarose?)


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Old 11-05-2004, 10:32 AM
Rafael Maldonado
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Default Nusieve agarose gel

Samantha wrote:
Nusieve is (was, at leas) a trademark of FMC; which probably have merged
in a bigger biotech company by now. It is special agarose for very small
DNA fragments. If you do not have it, you may use 3-4% "normal" agarose,
but it will not have the same resolution than Nusieve. Be careful, with
that agarose concentration I would not recommend microwave, but ina
boiling water bath.

Rafael Maldonado, Ph. D.
Division of Genetics
University of Alicante
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agarose , gel , nusieve

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