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How do I sterilize tiny volumes of solutions?

How do I sterilize tiny volumes of solutions? - Protocols and Methods Forum

How do I sterilize tiny volumes of solutions? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 10-29-2004, 02:48 PM
Paul Wary
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Default How do I sterilize tiny volumes of solutions?



Hello,

I have tiny volumes of solutions (around 100 Ál) that I need to
sterilize before use in cell culture. Autoclaving is no option because
the solvents are not stable enough. Filtration doesn't work with the
filters we have because they almost completely soak up the entire
volume during filtration. Are there any special filters for this
purpose or any other options for sterilizing such tiny volumes?

Paul
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  #2  
Old 10-30-2004, 03:00 AM
D.K.
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Default How do I sterilize tiny volumes of solutions?

On 29 Oct 2004 07:48:34 -0700, [Only registered users see links. ] (Paul Wary) wrote:


There are filters with retention of only 10 microL I use them regularly
for analytical HPLC and occasionally for sterilization.

This is catalog # for 0.2 u filters
[Only registered users see links. ]
but the picture is *wrong*. The filters in reality look like this:
[Only registered users see links. ]
(you can buy these and sterilize them with ethylene oxyde
yourself - this is what Millipore does anyway).

DK

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  #3  
Old 11-02-2004, 07:35 PM
Siddhartha S Mitra
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Default How do I sterilize tiny volumes of solutions?

Paul Wary wrote:


Anybody every tried using the microfuge membranes. I have used it once
and lost about 10ul
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