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Denatured alcohol (ethanol) OK for standard molecular biology solutions?

Denatured alcohol (ethanol) OK for standard molecular biology solutions? - Protocols and Methods Forum

Denatured alcohol (ethanol) OK for standard molecular biology solutions? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-18-2004, 01:23 PM
Paul Wary
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Default Denatured alcohol (ethanol) OK for standard molecular biology solutions?



Hi,

We had non-denatured alcohol (ca. 99 %, pro analysi) and denatured
alcohol (ca. 99 % with around 1 % methylethylketone, pro analysi) in
our lab. We were running out of non-denatured alcohol, and I asked one
my lab mates whether I can use the denatured alcohol for making
standard molecular biology/biochemistry solutions (such as certain
buffers for precipitation or washing of nucleic acids, washing
solution for silver staining of protein gels).
He said no, I would have to use non-denatured alcohol but couldn't
give me a reason for that. I can't think of a reason why 1 %
methylethylketone would be a problem for the uses of alcohol
mentioned above.
Can you tell me when denatured alcohol can be used for standard
procedures of molecular biology and biochemistry, when not, and why?

Paul
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Old 10-18-2004, 05:55 PM
D.K.
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Default Denatured alcohol (ethanol) OK for standard molecular biology solutions?

In article <[Only registered users see links. ] >, [Only registered users see links. ] (Paul Wary) wrote:

I think its perfectly safe to use denatured ethanol. 2-butanone is
pretty inert thing that will not react with anything in your sample.

DK
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