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His-tag like proteins in E. coli?

His-tag like proteins in E. coli? - Protocols and Methods Forum

His-tag like proteins in E. coli? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-28-2004, 03:13 PM
Peter Frank
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Default His-tag like proteins in E. coli?



Hello,

I used an anti-His-tag antibody in order to detect the His-tagged
protein that I expressed in E.coli (strain BL21 (DE3)). I also used a
negative control with no His-tagged protein expression and got some
bands there.

Are there any proteins naturally occuring in E.coli which contain
His-tag like segments that will bind anti-His-tag antibody?

Peter
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Old 09-28-2004, 05:12 PM
Louis Hom
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Default His-tag like proteins in E. coli?


this is a totally useless followup -- I apologize -- but yes, there is a
cellular protein that often copurifies with his-tagged proteins. I
unfortunatley can't remember what it's called, however.
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