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stripping antibodies from western blots

stripping antibodies from western blots - Protocols and Methods Forum

stripping antibodies from western blots - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-25-2004, 05:02 PM
Carl Ziess
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Default stripping antibodies from western blots



Dear all, I am in need of a good protocol for stripping only secondary
antibody (if possible) and primary+secondary antibodies from western blots.
This need arise from my mistakenly putting anti rabbit secondary on a blot,
and I like to see if this blot can be reused for probing a different
protein. Any expert input will be appreciated. Thank you.

Carl Ziess


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Old 09-26-2004, 05:31 AM
Don Incognito
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Default stripping antibodies from western blots

"Carl Ziess" <[Only registered users see links. ]> wrote in message news:<yEg5d.434$[Only registered users see links. ].prodigy.com >...

Assuming you haven't developed the blot, you don't need to strip it:
simply incubate with the correct secondary.

Don Incognito
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