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Cresol red for PCR loading buffer...

Cresol red for PCR loading buffer... - Protocols and Methods Forum

Cresol red for PCR loading buffer... - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 08-04-2004, 05:48 PM
jo
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Default Cresol red for PCR loading buffer...



Hi!

I´d like to prepare my own PCR loding buffer with cresol red.
Do I have to use the sodium salt or pure cresol red to solve it?
Which one gives the better results?
Any recipes???

best regards

J. Seggewiss


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  #2  
Old 08-04-2004, 10:25 PM
Wolfgang Schechinger
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Default Cresol red for PCR loading buffer...

As you have hydrophilic conditions, you'll probably be better off with the
salt. As CR is a pretty old compound, you might find some solubility data in
the Beilstein or the CRC handbook of organic chemicals, Or simply, check
Sigma (they should sell it) online for a data sheet and solubility data.

Please tell us

a) what makes Cresol red so interesting (never heard about it, but I am
really curious!)

b) if there is a reference

c) How you dissolved it in the end!

Best regards!

Wo


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