Cresol red for PCR loading buffer...

Hi!

I´d like to prepare my own PCR loding buffer with cresol red.
Do I have to use the sodium salt or pure cresol red to solve it?
Which one gives the better results?
Any recipes???

best regards

J. Seggewiss

As you have hydrophilic conditions, you'll probably be better off with the
salt. As CR is a pretty old compound, you might find some solubility data in
the Beilstein or the CRC handbook of organic chemicals, Or simply, check
Sigma (they should sell it) online for a data sheet and solubility data.

Please tell us

a) what makes Cresol red so interesting (never heard about it, but I am
really curious!)

b) if there is a reference

c) How you dissolved it in the end!

Best regards!

Wo


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