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RNA decomtamination solution

RNA decomtamination solution - Protocols and Methods Forum

RNA decomtamination solution - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 07-31-2004, 11:16 AM
Neil Horner
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Default RNA decomtamination solution



Hi

Does anyone know what is in the RNA decontamination solutions currently on
the market, such as BD biosciences RNA Blaster or Ambion's RNA zap.

Regards



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  #2  
Old 07-31-2004, 11:54 AM
Kevin M Jude
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Default RNA decomtamination solution

Neil Horner ([Only registered users see links. ].uk) wrote:
: Hi

: Does anyone know what is in the RNA decontamination solutions currently on
: the market, such as BD biosciences RNA Blaster or Ambion's RNA zap.

I don't know what is in those solutions, but my homemade solution is 0.1
M NaOH, 10 mM EDTA.

kmj

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  #3  
Old 07-31-2004, 12:08 PM
Neil Horner
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Default RNA decomtamination solution

Thanks, I'll try it

Neil
"Kevin M Jude" <[Only registered users see links. ].upenn.edu> wrote in message
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  #4  
Old 08-01-2004, 03:12 AM
Nick Theodorakis
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Default RNA decomtamination solution

On Sat, 31 Jul 2004 12:16:55 +0100, "Neil Horner"
<[Only registered users see links. ].uk> wrote:


I don't know those particular products, but just to clarify: are you
trying to get rid of RNA, or get rid of contaminants that may
interfere with RNA purification/analysis? If the former, RNA is very
base-labile, so getting rid of it is no problem with NaOH or something
similar.

Nick

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  #5  
Old 08-01-2004, 02:31 PM
Neil Horner
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Default RNA decomtamination solution

Hi, thanks for your reply. Sorry, I didn't make it clear in my original
post. I am looking for a solution that can be used to spray work surfaces
and pipettes etc., which will eliminate any RNase present

Cheers

Neil
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  #6  
Old 08-01-2004, 06:21 PM
Peter Ellis
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Default RNA decomtamination solution

[Only registered users see links. ] wrote:

Magma. Obtaining such, and developing a protocol which leaves the
surfaces usable afterwards, are left as an exercise for the grad
student.

Peter
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