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TUNEL reaction in paraffin sections

TUNEL reaction in paraffin sections - Protocols and Methods Forum

TUNEL reaction in paraffin sections - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 06-15-2004, 09:05 PM
moriah.nickerson@tufts.edu
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Default TUNEL reaction in paraffin sections



I am a first year graduate student who is interested in the development of the dopaminergic neurons in the substantia nigra. I have been trying to use paraffin-embedded sections to look at apoptotic events in the substantia nigra in early postnatal development. I have had inconsistant results with a high amount of background signal. I have tried a few methods and was hoping to get more suggestions. Does anyone have a good working protocol for TUNEL staining with paraffin sections?
Thank you!
Moriah

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