Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


problem-DNA extraction from paraffin sections

problem-DNA extraction from paraffin sections - Protocols and Methods Forum

problem-DNA extraction from paraffin sections - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-20-2004, 04:22 AM
gurukmc@hotmail.com
Guest
 
Posts: n/a
Default problem-DNA extraction from paraffin sections



Hello everybody,
Iam Guru prasad an Indian research scholar. Iam finding difficult in extraction of DNA from formalin fixed paraffin sections. I have tried different extraction methods mainly using phenol chloroform extraction and ethanol precipitation but I could not able to obtain DNA. Please help me to soughtout this problem
Thank you

Guru Prasad
[Only registered users see links. ]



[Only registered users see links. ]
Reply With Quote
  #2  
Old 05-20-2004, 04:37 PM
H@ns
Guest
 
Posts: n/a
Default problem-DNA extraction from paraffin sections

We have rather good experiences with Chelex 100, which is an ion
exchange resin. If you google for "chelex paraffin sections" you'll
come across numerous protocols.

Good luck!

Hans

On 20 May 2004 04:22:35 -0000, [Only registered users see links. ] wrote:


Reply With Quote
Reply

Tags
extraction , paraffin , problemdna , sections


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Plant Protein Extraction the Hard Way danfive Protein Science 0 04-24-2008 08:53 PM
Protein Extraction Problems molecule2005 Protein Science 0 04-16-2008 08:57 AM
Problem with extraction of DNA from Trizol max_rempel Epigenetics Forum: DNA Methylation, Histone and Chromatin Study 2 01-11-2008 03:39 PM
Problems with RNA extraction or QPCR for AI deki Real-Time PCR and Quantitative PCR Forum 2 12-30-2007 08:58 PM
Problems with RNA extraction or QPCR for AI deki Basic Lab Protocols and Techniques 3 07-27-2007 02:59 AM


All times are GMT. The time now is 04:25 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11494 seconds with 16 queries