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Agrobacterium EHA105

Agrobacterium EHA105 - Protocols and Methods Forum

Agrobacterium EHA105 - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 05-18-2004, 12:30 PM
Michael Witty
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Default Agrobacterium EHA105




Dear All,
I am trying to use the Wizard Miniprep kit to purify an approx 14kbp
plasmid from Agrobacterium EHA105 but have found only DHA far too big to
be my plasmid. It is strange for the following reasons

1]. It is high molecular weight DNA BUT does not stay near the well like
undigested chromosomal DNA

2]. The protocol worked will for Agrobacterium GV3101 but not for EHA105.
Is this strain to strain variation to be expected?

Comments gratefully received!

Regards, Mike.

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  #2  
Old 09-06-2009, 06:04 PM
Pipette Filler
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Default Re: Agrobacterium EHA105

Dear all
I want to know the proper protocol for the making chemical competent cells of agrobacterium EHA105 strains
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Old 12-31-2009, 12:45 PM
Pipette Filler
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Default Re: Agrobacterium EHA105

Dear all,

I am new in member of this site, so i am sorry if it is not the right place to ask.
I am looking for Agrobacterium tumefacuens strain EHA105, but i was unable to find a commercial supplier. Can i get some hepl? Thanks in advance

Samir
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Old 04-12-2010, 04:27 AM
Pipette Filler
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Default Re: Agrobacterium EHA105

dear
have u found EHA105?

Farah
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