Biorad Bradford Assay

I am using a Biorad Bradford Protein Assay to estimate total protein from
tissue lysates, fir westrn blot analysis. I use a dilution of 1:20 to 1:50
of the lysates to estimate protein. At this dilution I should not get any
interference in the assay from the SDS, NP40, and Na-Deoxycholate (present
in the tissue lysis buffer). However the estimation are not always
reproducible.
Does anyone have any tips/advice on this issue, please

Thanks

OK

In article <[Only registered users see links. ] m>, "OK" <[Only registered users see links. ]> wrote:

1) Work more carefully, be more accurate at all steps.
2) Try to take the spec reading at the same amount of time after
mixing protein into the reagent. About 10-20 min is optimal.
The absorbance does changes with time.

DK