Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Protocols and Methods Forum (http://www.molecularstation.com/forum/protocols-methods-forum/)
-   -   Biorad Bradford Assay (http://www.molecularstation.com/forum/protocols-methods-forum/17424-biorad-bradford-assay.html)

OK 05-07-2004 10:30 PM

Biorad Bradford Assay
 
I am using a Biorad Bradford Protein Assay to estimate total protein from
tissue lysates, fir westrn blot analysis. I use a dilution of 1:20 to 1:50
of the lysates to estimate protein. At this dilution I should not get any
interference in the assay from the SDS, NP40, and Na-Deoxycholate (present
in the tissue lysis buffer). However the estimation are not always
reproducible.
Does anyone have any tips/advice on this issue, please

Thanks

OK



D.K. 05-08-2004 12:32 AM

Biorad Bradford Assay
 
In article <[Only registered and activated users can see links. Click Here To Register...] m>, "OK" <[Only registered and activated users can see links. Click Here To Register...]> wrote:

1) Work more carefully, be more accurate at all steps.
2) Try to take the spec reading at the same amount of time after
mixing protein into the reagent. About 10-20 min is optimal.
The absorbance does changes with time.

DK


All times are GMT. The time now is 06:08 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08909 seconds with 11 queries