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homeemade thermocycler?

homeemade thermocycler? - Protocols and Methods Forum

homeemade thermocycler? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-28-2004, 09:19 AM
Wolfgang Schechinger
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Default homeemade thermocycler?



Dear all, just out of curiosity: Is there anyone who has built a
thermocycler?

Wo
Wolfgang Schechinger


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  #2  
Old 04-28-2004, 01:28 PM
Walter Schick - BioSepCo
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Default homeemade thermocycler?

Use several water baths, set at different temperatures and move a rack
between them. If you add a robot, you are recreating the principle of
Stratagene's system. Generally very fast reactions as there is no warm up
ramp or cool down ramp like on commercial block systems.

Best regards,

Walter Schick
Bio-Sep Company agent for Labhoo.com
[Only registered users see links. ]
----- Original Message -----
From: "Wolfgang Schechinger" <[Only registered users see links. ]>
To: <[Only registered users see links. ].ac.uk>
Sent: Wednesday, April 28, 2004 2:19 AM
Subject: homeemade thermocycler?



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  #3  
Old 04-29-2004, 12:27 PM
Louis Hom
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Default homeemade thermocycler?

In article <[Only registered users see links. ].net>,
Wolfgang Schechinger <[Only registered users see links. ]> wrote:

I looked into it about 4 or 5 years ago and it seems very do-able,
assuming you either know how to talk to the RS232 port or can program a
microprocessor. I didn't have time to learn either, but it doesn't sound
exceedingly difficult. I think you can probably build one for about $150
or less. You can do a search on google groups for "$100 thermocycler".
--
__________________________________________________ ____________________________
Lou Hom >K'93
[Only registered users see links. ]
[Only registered users see links. ]
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  #4  
Old 04-30-2004, 10:46 AM
Wolfgang Schechinger
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Default homeemade thermocycler?

Dear Walt,

that's a very good setup if you want or need to save money. A device like
this, constructed from very famous Fischertechnik tiles and operating with
3 aquariums filled with oil was used for diagnostic PCR in a hospital in
Munich in the 90s and later, when commercial thermocyclers wre not
available yet or at least horribly expensive. It was controlled with a
PC-XT running at 5 MHz and worked like a charm for many years.

Basically, I am looking for some ideas on how to construct a programmable
thermostate which is small, portable, does not consume a lot of energy and
does not make a lot of noise. And supports gradients.
Maybe there are some ideas or solutions on the net on how do heat and cool
the tubes and to control the temperature.

So, if you just need a PCR machine, you may go to ebay and buy a PE cetus
for 100 euros of course. I already have some ideas, but I don't want to
invent the wheel a second time. And I don't want to mis-invent a wheel with
3 or 4 corners.

Best regards,

Wo


At 14:28 28.04.2004 +0100, Walter Schick - BioSepCo wrote:

Wolfgang Schechinger


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