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real-time thermocyclers. Any recommendations?

real-time thermocyclers. Any recommendations? - Protocols and Methods Forum

real-time thermocyclers. Any recommendations? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-27-2004, 08:00 PM
Dr. James J. Campanella
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Default real-time thermocyclers. Any recommendations?



Dear List,

We are considering the purchase of a new real-time thermocycler. We are
looking at the Lightcycler and the ABI systems. The ABI's seem to be
cheaper, but we have experienced less than stellar performance from our ABI
sequencer and standard thermocycler and do not want to end up with another
expensive paperweight. The Lightcycler costs more by about $15,000, but is
rumored to be more accurate and flexible.

I am just curious if anyone out there has a specific or general
recommendation for these or any other system. This is a major high-end
purchase for us and we do not want to make a mistake that will end up
wasting our research dollars.

Thanks,

Jim


James J. Campanella,
Asst. Professor
Department of Biology and Molecular Biology
Montclair State University
1 Normal Avenue
Montclair, NJ 07043

Alternate email address: [Only registered users see links. ]

Ph: 973-655-4097
Fax: 419-791-9834

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Old 05-15-2004, 09:10 AM
brenda
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Default real-time thermocyclers. Any recommendations?

Dear James,

We are now using the LightCycler and the MJ in our lab. The last one is out
and away due to technical problems. In my hands the LightCycler is working
fine. It would be very nice, however, if the company would give more
accurate technical support, but that's just our problem.

Good luck!

ps: we have tried different machines during a 2-weeks demonstration. I would
suggest to organise this for your lab too. We had the I-Cycler (Bio-Rad),
Rotorgene (Corbett Research), Opticon (MJ) and LightCycler (unfortunately
not the 2.0 version) all together in the lab

""Dr. James J. Campanella"" <[Only registered users see links. ]> schreef in
bericht news:6.1.0.6.0.20040427154758.01bf6368@mail.montcl air.edu...
ABI


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