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Question abt gradient gel electrophoresis

Question abt gradient gel electrophoresis - Protocols and Methods Forum

Question abt gradient gel electrophoresis - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-22-2004, 02:00 AM
Shyam
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Default Question abt gradient gel electrophoresis



The question i have is, what is the size range of separation on gradient
gels.I want to use gradient gel to separate two molecules one has a size of
500 daltons while the other is about 110Kda. Is that possible? If so how?

From what i have looked up on the web, the closest answer i got to my
question is using a 15% gradient gel you can separate 2500 to 100000 da
molecules on these gels.

Thx in anticipation. Shyam.
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