Roche RTS Protein Expression

Hello,

I realize that this might be a little off-topic, but I thought some
readers might be interested in it anyway.

Thank you,
Evan Bursey

Mycobacterium Tuberculosis Structural Genomics Consortium
Li-Wei Hung Lab
Lawrence Berkeley National Laboratory

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Roche RTS Workshop
July 6-7, 2004
Lawrence Berkeley National Laboratory


The Mycobacterium Tuberculosis Structural Genomics Consortium and Roche
Applied Science are organizing a workshop to provide education and
hands-on training for the Roche RTS Cell-free Protein Expression
System. The workshop will be held on July 6-7, 2004 at the Lawrence
Berkeley National Laboratory in Berkeley, CA.

Highlights of the workshop will include:

* Tutorial on template generation, followed by hands-on training.
* Tutorial on protein expression using RTS E. coli 100 HY, RTS 500 HY,
RTS 9000 HY, followed by hands-on training.
* Discussion of Roche's ProteoExpert system, followed by hands-on
training.
* Introduction to RTS Wheat Germ System and RTS E. coli Disulfide kit.
* Troubleshooting (scale-up, protein detection, linear template
generation).
* Participants may use DNA provided by Roche, or they may provide their
own plasmid DNA and express their own protein.

Enrollment is limited to just 12 individuals. Preference will be given
to participants from NIH-supported structural genomics centers and to
industrial scientists. Interested participants should have a basic
understanding of molecular biology and should possess basic computer
skills.

The cost for academic participants is $100. The cost for non-academic
participants is $1200.

The preregistration deadline for participants who wish to provide their
own plasmid DNA is April 19, 2004. Plasmid sequence information must be
provided to Roche by April 21, 2004. All such information will be
treated as confidential and will not be made available to any other
party. A sample of the plasmid must be provided to Roche by May 3,
2004. The deadline for participants not providing their own plasmid DNA
is April 30, 2004. To preregister, please send email to Evan Bursey
([Only registered users see links. ]) with the following information: name, institution,
PI, and whether or not you wish to provide your own expression target.



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