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TLC protocols

TLC protocols - Protocols and Methods Forum

TLC protocols - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 04-14-2004, 02:53 PM
Sean Patterson
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Default TLC protocols



Does anyone know of a Web resource listing TLC solvent systems for
separating different lipid classes? I've been Googling without much
luck - either nothing or too many hits depending on the query.

Sean Patterson
Universidad Nacional de Cuyo
Waaayyy down South
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  #2  
Old 04-14-2004, 05:10 PM
Wolfgang Schechinger
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Default TLC protocols

Sean,

try to find a copy of "Thin Layer Chromatography" by Stahl. There you'll
find lots of basic receipes. If you have a more specialised problem, check
PubMed or simply ask here in the Bionet.
Wo

At 15:53 14.04.2004 +0100, Sean Patterson wrote:
Wolfgang Schechinger


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  #3  
Old 04-14-2004, 05:36 PM
Sean Patterson
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Default TLC protocols

Hi Wolfgang.

I was sure I had a photocopy of the relevant chapter from Stahl,
but I'm unable to find it in my rather chaotic filing system and the
libraries here in Mendoza (Argentina) don't carry a copy. The medline
search turns up about 500 hits, most from pre-pdf days, which brings
me back to the limited library problem.

My specific problem is that I have an old vial (7 years) of
3H-palmitic acid (5mCi/ml) in ethanol, and I want to know if I need
to repurify it before use. I suppose the most probable chemical
contaminant would be the formation of ethyl-palmitate over time, but
I don't know what the radiolytic breakdown would produce.

If no-one has any experience with this, I'll see if I have
anyluck with NEN tech service. They'll probably just tell me to buy
some more.

Sean



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  #4  
Old 04-16-2004, 09:45 AM
Dr Engelbert Buxbaum
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Default TLC protocols

Sean Patterson wrote:




If you have no luck with the Stahl, your best bet might be to use a
reversed phase matrix similar to the ones used in HPLC. A check for
"fatty acid" and "HPLC" might turn up something that can be adapted to
the TLC-format.

Note that for your application you will probably have to add carrier
palmitic acid to the sample and check by fluorography that the
radioactivity coincides with the palmitate spot.
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