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RACE PCR Kit Recommendations Please

RACE PCR Kit Recommendations Please - Protocols and Methods Forum

RACE PCR Kit Recommendations Please - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 04-13-2004, 03:59 PM
Stefan
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Default RACE PCR Kit Recommendations Please



Hi,

I would like to do some RACE PCR in the near future. I've looked at
some of the RACE PCR kits that are available, and they all seem to
have two things in common - high prices and low number of reacions.
That means that if I can convince my boss to purchase a kit, I will
have very few chances to mess it up before getting it right. So what
I am looking for are some recommendations.

I would like to do 5' RACE, but wouldn't mind having the option of
doing 3' RACE. I would be especially interested in experiences from
those of you working on Arabidopsis.

Thanks in advance,

Stefan
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Old 04-16-2004, 09:15 AM
tvink
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Default RACE PCR Kit Recommendations Please

HI,

We tried several kits / methods and in our hands the SMART RACE
technology of BD is the best (no affiliation). It is expemnsive but it
works most of the time for 5'RACE (we never tried 3' RACE). You can
scale down the recommended volumes down to 5 microl. If you wanna do
lots of RACES you can even order their smart RACE oligo custom made to
bring down the costs. Hope this helps.


Grtz Tom


On 13 Apr 2004 08:59:47 -0700, [Only registered users see links. ] (Stefan)
wrote:


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