Alternatives to Laemli?

Several years ago, I wanted to run a gel at lower pH to maintain an ester linkage between 2 proteins (see Sullivan and Vierstra, J. Biol. Chem. 268:8777) A colleague suggested the method in the following reference:

Weber, K, and Osborn, M (1969) The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406-4412.

The gel gets run at pH 7.2 in a phosphate buffer. Since the original reference is old, it used tube gels, but we adapted it to run slab gels. Since there is no stack with this one, sample volumes need to be small to get tight bands.

I hope this helps.

Mike Sullivan
Research Molecular Geneticist
USDFRC-ARS-USDA


----- Original Message -----
From: [Only registered users see links. ]
Date: Monday, February 16, 2004 10:25 am
Subject: Alternatives to Laemli?


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In article <[Only registered users see links. ].edu>,
[Only registered users see links. ] (MICHAEL L SULLIVAN) wrote:

There is no reason not to include the stacker.