Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


FW: DNA fingerprinting for yeast

FW: DNA fingerprinting for yeast - Protocols and Methods Forum

FW: DNA fingerprinting for yeast - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-06-2004, 03:48 PM
Deanne Bell
Guest
 
Posts: n/a
Default FW: DNA fingerprinting for yeast





-----Original Message-----
From: [Only registered users see links. ] [mailtowner-methods@hgmp.mrc.ac.uk]
On Behalf Of [Only registered users see links. ]
and followed their recommended PCR conditions. But after running the
gel electrophoresis, i can only obtain smear for each lane, which were
loaded with the amplicons, instead of getting some bands.



SMEARS USUALLY ARE DUE TO NON-SPECIFIC ANNEALING OF THE PRIMERS DURING
PCR.
SOME IDEAS TO START YOU OFF:
1) OPTIMIZE MgCl BY RUNNING A GRADIENT OF REACTIONS RANGING FROM
1mM - 6mM FINAL CONCENTRATION.

2) OPTIMIZE ANNEALING TEMPERATURE OF PCR

3) USE A 'HOT START' PCR METHOD

4) MAKE SURE YOU ARE USING GEL % THAT IS OPTIMAL FOR THE LENGTH OF
YOUR PCR PRODUCT
---
Reply With Quote
Reply

Tags
dna , fingerprinting , yeast


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
DNA Fingerprinting Umar Tofa Molecular Biology Techniques 0 05-27-2010 06:02 PM
Bands fade in ISSR fingerprinting kashir Agarose Gel Electrophoresis Forum 2 03-01-2010 06:13 AM
Gel electrophoresis (used in DNA fingerprinting) separates DNA fragments duvalballer21 DNA Techniques 0 08-06-2009 05:15 PM
Genetic Fingerprinting... jax Biology Forum 3 01-03-2009 07:09 AM
DNA fingerprinting for yeast dav668@yahoo.com Protocols and Methods Forum 0 02-06-2004 01:54 AM


All times are GMT. The time now is 01:00 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11104 seconds with 16 queries