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DNA fingerprinting for yeast

DNA fingerprinting for yeast - Protocols and Methods Forum

DNA fingerprinting for yeast - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-06-2004, 01:54 AM
dav668@yahoo.com
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Default DNA fingerprinting for yeast



hi,
I have been doing DNA fingerprinting for the past few months by using RFLP method. I used a primer, which had been published on some journals, and followed their recommended PCR conditions. But after running the gel electrophoresis, i can only obtain smear for each lane, which were loaded with the amplicons, instead of getting some bands. I suspected my primer had degraded, thus i changed to another stock again. But the problem is still remain.

Please advise. Thanks.

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