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DNA fingerprinting for yeast
I have been doing DNA fingerprinting for the past few months by using RFLP method. I used a primer, which had been published on some journals, and followed their recommended PCR conditions. But after running the gel electrophoresis, i can only obtain smear for each lane, which were loaded with the amplicons, instead of getting some bands. I suspected my primer had degraded, thus i changed to another stock again. But the problem is still remain.
Please advise. Thanks.
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|dna , fingerprinting , yeast|
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