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Retroviral promoter : next to my Promoter

Retroviral promoter : next to my Promoter - Protocols and Methods Forum

Retroviral promoter : next to my Promoter - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 02-03-2004, 04:46 AM
Tobias McNultey
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Default Retroviral promoter : next to my Promoter



I'm looking at this gene that has interesting
transcription properties. I have
the genomic sequence near the 5' end of
the transcriptional strat site.

A promoter finder found this sequence which
has CCATC and TATAAAA and a GC box--real pretty canonical promoter
but it's an "MLT1" repeat about 200 nucs upstream
of my transcr start site and same orientation 5' to 3'.
Another (predicted)promoter lies closer to the transcr start site,
maybe the "real" prmoter, but less typical-looking.

Might this promoter-like seq play a role in the transcription of
my gene? If it's such a canonical looking promoter,
might it interfere? The chimp ortholog doesn't have this retroviral
promoter in its sequence but only human does.
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Old 02-03-2004, 06:08 AM
P.C.
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Default Retroviral promoter : next to my Promoter

everything is possible; at this level of detail you really have to do an
experiment (often to realize that all you were guessing before was wrong).

retroviruses have played their role in the evolution (and continue to do
so), expression of your gene might have been affected in humans.

did you try mouse and fish?


Peter


Tobias McNultey wrote:

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