hello, I'm new here and also I've just begun to work in the laboratory. My boss is pretty demanding and I have a lot of problems and doubts. could you please help me with (i guess simple) calculations?
i'm supposed to do in vitro experiment. i need to use my oligos as usually (0,03 pmol/assay) and add some inhibitors (peptides) in a final concentration 100nM. My stock of inhibitors is 5,5 mikroM. For one assay i use volume of 10 mikrol. How much of inhibitor should I add?
Thank you very much for help in advance.