Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

supershift of gel shift assay

supershift of gel shift assay - Protocols and Methods Forum

supershift of gel shift assay - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 12-10-2003, 11:05 AM
Takeshi Miura
Posts: n/a
Default supershift of gel shift assay

Nice to meet you every one!!

Sameone mentioned protocol about gel shift assay.
I can see specific DNA-protein complex band in gel shift assay.
And, i want to identify the protein binding oligo DNAprobe.
Now, i expect it is one protein. So, now try supershift assay using
this protein specific antibody.
But, no any change between control(no antibody) lane and supershift lane.
my protocol about super shift reaction is...

protein extract 2ug and PolyL etc.
30min on ice
add antibody
30min on ice
aplly, electrophoresis using 8% PAGE
and detection by DIG system(non-RI system)

After added antibody, 30min reaction time is enough?
on ice is adequat?
if depend on type of protein, please teach me about your good protocols!
Thank you!!

Reply With Quote

assay , gel , shift , supershift

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA EMSA Mobility Shift Assay gongdonas RNA Techniques Forum 4 05-15-2008 10:01 PM
Optimizing RNA EMSA Electrophoretic Mobility Shift Assay? eyesyah84 Electrophoretic Mobility Shift Assay Forum 1 04-13-2008 12:34 AM
Gel shift assay FM07 Protocols and Methods Forum 2 01-31-2008 09:05 PM
DNA EMSA electrophoretic mobility shift assay darkwng DNA Techniques 0 07-10-2006 04:56 AM
Biorad Bradford Assay Deanne Bell Protocols and Methods Forum 0 05-13-2004 03:21 PM

All times are GMT. The time now is 04:50 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11704 seconds with 16 queries