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No subject was specified. - Protocols and Methods Forum

No subject was specified. - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 12-02-2003, 01:51 PM
Laura Segall
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Default No subject was specified.



forgot to mention that I did try the positive control--it works.
I will try changing the template concentration too.

Thank you,

Laura
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Old 12-02-2003, 09:10 PM
Wolfgang Schechinger
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Default Quick Change Woes

Laura,

maybe the primers just don't align - did you lower the annealing temp and /
or increase the magnesium concentration?
For the first issue, a gradient cycler or a touch down PCR could be useful.

Wo

At 13:51 02.12.2003 -0000, Laura Segall wrote:

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