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#1
12-02-2003, 12:51 PM
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 No subject was specified. forgot to mention that I did try the positive control--it works. I will try changing the template concentration too. Thank you, Laura ---  |
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#2
12-02-2003, 08:10 PM
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| Quick Change Woes Laura, maybe the primers just don't align - did you lower the annealing temp and / or increase the magnesium concentration? For the first issue, a gradient cycler or a touch down PCR could be useful. Wo At 13:51 02.12.2003 -0000, Laura Segall wrote: --- |
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