Plasmid extraction kits

When I used to use quiagen mini prep kits during my PhD program back in India, our lab members often used to use the columns from the older kits in the place of ethanol precipitation. ACtually most plasmid extracts kits essentially employ the same principles of alkaline lysis. I still think alkaline lysis (done properly), is the best method to be used for plasmid preparation. I always used to get more DNA prep from alkaline lysis than from any other method.

jai



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Hi,

take a look at the CTAB protocol. A boiling lyis followed by a CTAB
precipitation. Used to be my favorite before the kits. Fast and good
DNA.

[1] G. Del Sal, G. Manfioletti and C. Schneider, The CTAB-DNA
precipitation method: A common mini-scale preparation of template DNA
from phagemids, phages or plasmids suitable for sequencing,
Biotechniques, 7 (1989), pp. 514-520.

grtx Tom



On 26 Nov 2003 18:38:01 -0000, [Only registered users see links. ]
("Jayakumar, R") wrote:

[Joegreen]

I think all depends on the downstream application you want to use your DNA for