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Gel imaging systems

Gel imaging systems - Protocols and Methods Forum

Gel imaging systems - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 11-19-2003, 06:32 PM
chmc
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Default Gel imaging systems



Hi!

We're looking to buy a new digital gel imaging system (just something to
take pictures of gels, no fancy 2d gel analysis or anything), and I'm
wondering if anyone has any recommendation. Right now we have the UVP
digidocit system, and it is TERRIBLE! We've had nothing but trouble with it
from day one. I am anxious that our next one be at least decent.




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  #2  
Old 11-20-2003, 11:28 AM
Wolfgang Schechinger
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Default Gel imaging systems

What about a zoomable webcam or digital camera with a TWAIN interface, an
orange filter and an old PC? 500 MHz should be sufficient by far. Suggested
software: XNVIEW (freeware) or Paintshop Pro.
If you need video prints, use a b/w video camera attached to a frame
grabber / TV card with video in. The video printer may be looped through.

If you plan to buy a dedicated system, make sure it can export common
picture file formats with non-destructive compression like gif or tif on
disk. I have been using a geldoc system from Amersham (sorry, don't
remember the exact name) attached to a PC with dedicated software which
worked pretty well for me.

Wo


At 10:32 19.11.2003 -0800, chmc wrote:

---
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  #3  
Old 11-21-2003, 12:44 PM
Bob Hartley
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Default Gel imaging systems

[Only registered users see links. ] (Wolfgang Schechinger) wrote in message news:<[Only registered users see links. ].net >...

Hi there

I agree with wolfgang entirely.

Many software companies sel you a system that is really just a pixel
density routine.

If you use coloured marker bands then a good colour camera is
required. If greyscale there are many cheap CCD cameras available. A
good graphics card 50 pounds/dollars with frame capture is all you
need.

you can make your own lightbox.

In addition there are many good pieces odf freeware that will do this.

Imagetool
[Only registered users see links. ]
GIMP [Only registered users see links. ]
or ImageJ [Only registered users see links. ] are all you should need


Cheers
Bob: Sunny Scotland

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  #4  
Old 11-23-2003, 12:46 PM
BjornP
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Default Gel imaging systems

I have worked with several of the KODAK cameras, EDAS120, EDAS290, Gel Logic
100, the software is the same, KODAK 1D.
They work fine.
Check them out at this link:
[Only registered users see links. ]

BjornP

"chmc" <[Only registered users see links. ]> skrev i melding
news:bpgd1f$mpi$[Only registered users see links. ]...
it


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  #5  
Old 01-04-2004, 08:46 PM
Amanda
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Default Gel imaging systems

I would really REALLY recommend BioRad's GelDoc systems. Get them to do a
demo for you - wow!
Amanda


"chmc" <[Only registered users see links. ]> wrote in message
news:bpgd1f$mpi$[Only registered users see links. ]...
it


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