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Testing real-time PCR results for statistical significance?

Testing real-time PCR results for statistical significance? - Protocols and Methods Forum

Testing real-time PCR results for statistical significance? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 10-30-2003, 06:20 PM
Peter Frank
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Default Testing real-time PCR results for statistical significance?



How can I test my real-time PCR results for statistical significance?

I would like to judge statistically whether a rather small change in
expression between two samples is significant or not. I could do that
calculating a p value using a number of statistical tests. In this
regard two questions arise:
* Which statistical test should I use?
* To which data should I apply this test? The original Ct values, the
Ct value differences, the normalized expression levels?

Peter
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  #2  
Old 10-30-2003, 08:52 PM
Duncan Clark
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Default Testing real-time PCR results for statistical significance?

Historians believe that in newspost
<[Only registered users see links. ]> on Thu, 30 Oct 2003,
Peter Frank <[Only registered users see links. ]> penned the following literary
masterpiece:

Try asking the people on the qpcrlistserver on yahoogroups.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.
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  #3  
Old 10-30-2003, 09:36 PM
Peter Frank
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Default Testing real-time PCR results for statistical significance?

Duncan Clark wrote:


Thanks for the advice. I'll do just that. Didn't know about this Yahoo
group.

Peter
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pcr , realtime , results , significance , statistical , testing


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