Testing real-time PCR results for statistical significance?

How can I test my real-time PCR results for statistical significance?

I would like to judge statistically whether a rather small change in
expression between two samples is significant or not. I could do that
calculating a p value using a number of statistical tests. In this
regard two questions arise:
* Which statistical test should I use?
* To which data should I apply this test? The original Ct values, the
Ct value differences, the normalized expression levels?

Peter

Historians believe that in newspost
<[Only registered users see links. ]> on Thu, 30 Oct 2003,
Peter Frank <[Only registered users see links. ]> penned the following literary
masterpiece:

Try asking the people on the qpcrlistserver on yahoogroups.

Duncan
--
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

Duncan Clark wrote:


Thanks for the advice. I'll do just that. Didn't know about this Yahoo
group.

Peter