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| I currently use CTAB extraction for my genomic DNA extractions for southern, but I was wondering if anyone had tried using the Puregene kit from Gentras Systems. I use this kit in a multi-well format for small scale DNA extraction for PCR and Quantitative PCR. I recently visited Gentra's web page and found a protocol for a much larger scale prep. [Only registered users see links. ] if you are interested. This is a very simple protocol and very fast. As I said before, I have used DNA extracted using this protocol in PCR applications, so I'm assuming it would be servicable for southern use as well. The above protocol states a 90-900uG genomic yield, this is pleny for southern. Thanks for your help Nate |
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| dna , extraction , genomic , plant , southern |
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