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Plant Genomic DNA extraction for southern

Plant Genomic DNA extraction for southern - Protocols and Methods Forum

Plant Genomic DNA extraction for southern - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-15-2003, 04:59 PM
BioNate
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Default Plant Genomic DNA extraction for southern



I currently use CTAB extraction for my genomic DNA extractions for
southern, but I was wondering if anyone had tried using the Puregene
kit from Gentras Systems. I use this kit in a multi-well format for
small scale DNA extraction for PCR and Quantitative PCR. I recently
visited Gentra's web page and found a protocol for a much larger scale
prep.

[Only registered users see links. ] if you are interested.

This is a very simple protocol and very fast. As I said before, I have
used DNA extracted using this protocol in PCR applications, so I'm
assuming it would be servicable for southern use as well. The above
protocol states a 90-900uG genomic yield, this is pleny for southern.

Thanks for your help

Nate
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dna , extraction , genomic , plant , southern


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