Plant Genomic DNA extraction for southern
I currently use CTAB extraction for my genomic DNA extractions for
southern, but I was wondering if anyone had tried using the Puregene
kit from Gentras Systems. I use this kit in a multi-well format for
small scale DNA extraction for PCR and Quantitative PCR. I recently
visited Gentra's web page and found a protocol for a much larger scale
[Only registered and activated users can see links. Click Here To Register...] if you are interested.
This is a very simple protocol and very fast. As I said before, I have
used DNA extracted using this protocol in PCR applications, so I'm
assuming it would be servicable for southern use as well. The above
protocol states a 90-900uG genomic yield, this is pleny for southern.
Thanks for your help
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