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#1
10-13-2003, 05:21 AM
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 Southern hybridisation... How does SDS work in preventing non-specific blocking agent in hybridisation reactions? And what is the ideal conc? thanks. [Only registered users see links. ]  |
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#2
10-15-2003, 07:01 PM
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| Southern hybridisation... Hi, We have been using a simple 7.5% SDS / 5X SSPE as a hybridization reagent for years (no other additives). No background problems. It needs to be warmed to 65C to go into solution and is a bit thick, but other than that, it works great. Note: we use a positively charged nylon membrane, I'm not sure if it works as well with nitrocellulose. James Kami Department of Agronomy and Range Science University of California One Shields Avenue Davis, CA 95616-8515 USA email: [Only registered users see links. ] Tel: (530) 752-9982 Fax: (530) 752-4361 [Only registered users see links. ] wrote: |
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