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Southern hybridisation...

Southern hybridisation... - Protocols and Methods Forum

Southern hybridisation... - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-13-2003, 05:21 AM
ttanya_78@hotmail.com
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Default Southern hybridisation...



How does SDS work in preventing non-specific blocking agent in hybridisation reactions? And what is the ideal conc? thanks.


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Old 10-15-2003, 07:01 PM
Jim Kami
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Default Southern hybridisation...

Hi,
We have been using a simple 7.5% SDS / 5X SSPE as a hybridization
reagent for years (no other additives). No background problems. It needs
to be warmed to 65C to go into solution and is a bit thick, but other
than that, it works great.
Note: we use a positively charged nylon membrane, I'm not sure if it
works as well with nitrocellulose.

James Kami
Department of Agronomy and Range Science
University of California
One Shields Avenue
Davis, CA 95616-8515 USA
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Tel: (530) 752-9982
Fax: (530) 752-4361

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