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How does SDS work in preventing non-specific blocking agent in hybridisation reactions? And what is the ideal conc? thanks.
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We have been using a simple 7.5% SDS / 5X SSPE as a hybridization
reagent for years (no other additives). No background problems. It needs
to be warmed to 65C to go into solution and is a bit thick, but other
than that, it works great.
Note: we use a positively charged nylon membrane, I'm not sure if it
works as well with nitrocellulose.
Department of Agronomy and Range Science
University of California
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[Only registered users see links. ] wrote:
|hybridisation , southern|
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