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PCR primers for p53 in tumor cells?

PCR primers for p53 in tumor cells? - Protocols and Methods Forum

PCR primers for p53 in tumor cells? - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 10-01-2003, 05:06 PM
Peter Frank
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Default PCR primers for p53 in tumor cells?



I want to design PCR primers for p53 in a tumor cell line. I read that
p53 is often mutated in tumor cell lines, so how can I make sure or at
least increase the chances that my primers will work for this purpose?
After all, my template will be the human standard mRNA sequence for
p53 (accession number K03199).

Peter
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cells , p53 , pcr , primers , tumor


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