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Detection of DNA on a PVDF membrane

Detection of DNA on a PVDF membrane - Protocols and Methods Forum

Detection of DNA on a PVDF membrane - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-27-2003, 03:01 AM
EK
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Default Detection of DNA on a PVDF membrane



One more question for you guys. As I mentioned before, I made a gel shift in
native PAAG, and subsequently transferred both DNA and protein to the PVDF
membrane (Immobilon P). Both 5'-32P-labelled DNA and protein seem to stick
pretty well to membrane. Now, I want to determine whether anywhere on a
membrane there is a non-radioactive DNA (dephosphorylated as a result of
possible activity of the protein). The DNA is single stranded, and its
quantity is not very high (around 1 ng per lane). Is there a method of
staining the ssDNA on a membrane so that this 1 ng could be subsequently
visualized (specifically, to distinguish from protein)?
Cheers,
Emir


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