Detection of DNA on a PVDF membrane
One more question for you guys. As I mentioned before, I made a gel shift in
native PAAG, and subsequently transferred both DNA and protein to the PVDF
membrane (Immobilon P). Both 5'-32P-labelled DNA and protein seem to stick
pretty well to membrane. Now, I want to determine whether anywhere on a
membrane there is a non-radioactive DNA (dephosphorylated as a result of
possible activity of the protein). The DNA is single stranded, and its
quantity is not very high (around 1 ng per lane). Is there a method of
staining the ssDNA on a membrane so that this 1 ng could be subsequently
visualized (specifically, to distinguish from protein)?
|All times are GMT. The time now is 11:31 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved