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Bad Northern Background - Protocols and Methods Forum

Bad Northern Background - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 09-26-2003, 08:04 PM
Rajshree Mewani
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Default Bad Northern Background



I have to do northerns on a regular basis and many of them have been
successful, but recently I've been getting horrible background splotches
that are ruining my results. I've tried everything from starting over
with new RNA, new probes, new membranes and new hybridization solution
to having my boss watch my every step. Still nobody can figure out
what's going wrong. What makes it even more frustrating is that
sometimes, out of the blue, the blot will come out perfect even when I
followed the same protocol that previously gave bad results.
Does anyone have a clue as to what I should do???

Malika

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  #2  
Old 09-26-2003, 10:18 PM
Dr. Hiranya S. Roychowdhury
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Protocol please! It is hard to suggest remedy without knowing the protocol,
since different ones have different snags. BTW, try the Church's method. I
can almost guaruntee you will not do it any other way after that (unless you
are already there).

Quoting Rajshree Mewani <[Only registered users see links. ]>:



--
Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
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