Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

Bad Northern Background

Bad Northern Background - Protocols and Methods Forum

Bad Northern Background - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.

LinkBack Thread Tools Display Modes
Old 09-26-2003, 08:04 PM
Rajshree Mewani
Posts: n/a
Default Bad Northern Background

I have to do northerns on a regular basis and many of them have been
successful, but recently I've been getting horrible background splotches
that are ruining my results. I've tried everything from starting over
with new RNA, new probes, new membranes and new hybridization solution
to having my boss watch my every step. Still nobody can figure out
what's going wrong. What makes it even more frustrating is that
sometimes, out of the blue, the blot will come out perfect even when I
followed the same protocol that previously gave bad results.
Does anyone have a clue as to what I should do???


Reply With Quote
Old 09-26-2003, 10:18 PM
Dr. Hiranya S. Roychowdhury
Posts: n/a
Default Bad Northern Background

Protocol please! It is hard to suggest remedy without knowing the protocol,
since different ones have different snags. BTW, try the Church's method. I
can almost guaruntee you will not do it any other way after that (unless you
are already there).

Quoting Rajshree Mewani <[Only registered users see links. ]>:

Hiranya S. Roychowdhury, Ph.D.
Coll. Asst. Professor,
Molecular Biology,
Dept. of Chemistry & Biochemistry
Rm# 336, Chemistry Bldg.; MSC 3MLS
New Mexico State University
Las Cruces, NM 88003
Reply With Quote

background , bad , northern

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Western Blot Background Troubleshooting molecule2005 Western Blot Forum 18 10-07-2010 11:49 AM
Northern Blot Background and Non-Specific Probe Binding Ekn2484 RNA Techniques Forum 0 01-22-2009 08:16 PM
Big Bang's Afterglow Fails an Intergalactic Shadow Test In Fo Physics Forum 3 10-09-2006 01:03 PM
CDP-star and high background Jayakumar, R Protein Forum 1 02-09-2004 03:27 PM

All times are GMT. The time now is 06:34 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11371 seconds with 16 queries