I have to do northerns on a regular basis and many of them have been
successful, but recently I've been getting horrible background splotches
that are ruining my results. I've tried everything from starting over
with new RNA, new probes, new membranes and new hybridization solution
to having my boss watch my every step. Still nobody can figure out
what's going wrong. What makes it even more frustrating is that
sometimes, out of the blue, the blot will come out perfect even when I
followed the same protocol that previously gave bad results.
Does anyone have a clue as to what I should do???