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Reusing 96-well PCR plates

Reusing 96-well PCR plates - Protocols and Methods Forum

Reusing 96-well PCR plates - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 09-19-2003, 04:06 PM
Eric Vallender
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Default Reusing 96-well PCR plates



Has anyone had any luck cleaning out and reusing 96-well PCR plates? Our
lab goes through quite a bit of these and we are looking ot cut down on
their consumption. Specifically we are using the LabSource plates but
would be willing to change if it matters.

Thanks,

Eric Vallender
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Old 09-19-2003, 07:33 PM
Wolfgang Schechinger
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Default Reusing 96-well PCR plates

Eric,

as long as they don't have any special coating (I don't think that), the
most important thing is to get rid of DNA in the wells. After flushing them
with water, immerse the plates into bleach (NaOCl/NaOH, 5 to 10% should do
it, household bleach is ok) over night. Then just rinse well with dd water
afterwards and let them dry.

Best,

Wo

At 11:06 19.09.2003 -0500, Eric Vallender wrote:

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