Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Amplification of 2nd PCR product

Amplification of 2nd PCR product - Protocols and Methods Forum

Amplification of 2nd PCR product - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-02-2003, 04:19 PM
lecter
Guest
 
Posts: n/a
Default Amplification of 2nd PCR product



I am using a "two step PCR" method in order to generate point mutation
in plasmid DNA. Recently I have encountered into a strange problem.
By trying to amplify the 2nd PCR product with the two exterior primers
I get an unrecognized fragment, which differs in length of the 2nd PCR
product!
The reason I have tried to amplify the 2nd PCR product was a low yield
of the expected fragment, probably due to a formation of by-product
identical in length to the by-product received from the
"amplification" attempt.

What is the cause to this phenomenon?

Thanks

Gil Leichner
The institute of Lipid research
Sheba Medical Center
Israel
Reply With Quote
Reply

Tags
2nd , amplification , pcr , product


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Smearing-when PCR product is used as template tannamal@canr.cag.uconn.edu Protocols and Methods Forum 10 12-28-2010 07:48 AM
Weird amplification curve for Taqman probes fen Real-Time PCR and Quantitative PCR Forum 0 01-25-2010 09:35 AM
Product detectible on gel, melting curve peak, but no amplification curve emucaki Real-Time PCR and Quantitative PCR Forum 4 08-24-2009 08:54 PM
Amplification product size nmesbah@uga.edu Microbiology Forum 0 05-27-2004 07:17 PM


All times are GMT. The time now is 07:41 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10608 seconds with 16 queries