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Bionet posting (problems w/ chemiluminescence)

Bionet posting (problems w/ chemiluminescence) - Protocols and Methods Forum

Bionet posting (problems w/ chemiluminescence) - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-27-2003, 10:03 PM
Sean Patterson
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Default Bionet posting (problems w/ chemiluminescence)



>Dear Sean,

Surprisingly enough, I think this actually was a problem previously
when I was using Triton X-100 as the detergent. I was aware that
polyether detergents could breakdown and produce peroxides, and my
TX100 stock was getting old. Changing from the TX100 to the Tween
actually improved the signals, although I then started with
background problems that the Triton was solving - the original reason
to use it.

Since identifying the milk as a contributor to the problem I've gone
back over my lab book and confirmed that the problems did become
worse and more frequent after starting with it. I wonder if the
problem is the Argentine brand we're using.

Sean
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