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Bionet posting (problems w/ chemiluminescence)

Bionet posting (problems w/ chemiluminescence) - Protocols and Methods Forum

Bionet posting (problems w/ chemiluminescence) - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 08-27-2003, 02:55 PM
Sean Patterson
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Default Bionet posting (problems w/ chemiluminescence)



>Dear Sean,

Interesting suggestion, and I have considered it in the past in the
context of quantitation. Given the number of different proteins that
I measure, it would be difficult to achieve on a regular basis.


I have done this with my latest blot.


My washes are 5x5 minutes with PBS/Tween (0.2% v/v).


The transfers are usually done for 2 hours, and I often check the
progress on the readouts.


I have used several different power supplies.


I've done that too. Not always, but I do always stain/destain the
membrane with Ponceau S to confirm transfer.


Thanks for the suggestions

Sean
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