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Problems with chemiluminescence
Could it be the Tween-20 concentration. If concentrations of this go up more than the recommended (normally 0.05% for PVDF), I have heard of blots getting such clear backgrounds since they strip proteins from the blots. A drying step before blotting would be a good idea to improve protein binding.
I knew of a person in an earlier lab I worked with who used to add T-20 as per his estimation and not using exactly the amount required. he used to have very inconsistent results till we identified his problem.
But still a detailed protocol with timings and volumes would help.
|chemiluminescence , problems|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Problems with chemiluminescence||Jayakumar, R||Protocols and Methods Forum||6||08-27-2003 10:40 PM|
|Bionet posting (problems w/ chemiluminescence)||Sean Patterson||Protocols and Methods Forum||0||08-27-2003 10:03 PM|
|Problems with chemiluminescence||EK||Protocols and Methods Forum||1||08-26-2003 06:32 PM|
|Problems with chemiluminescence||Sean Patterson||Protocols and Methods Forum||1||08-25-2003 04:57 PM|