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polyA RNA isolation

polyA RNA isolation - Protocols and Methods Forum

polyA RNA isolation - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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  #1  
Old 08-24-2003, 06:42 PM
ip singh
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Default polyA RNA isolation



Hi all!
Could someone tell me the best working method to
isolate polyA RNA from Arabidopsis tissue? I need
polyA RNA in the order of about 50ug and I would not
mind buying appropriate kit available.
Thanks a lot.
IP

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  #2  
Old 08-25-2003, 07:56 AM
Wolfgang Schechinger
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Default polyA RNA isolation

Dear Ip,

the crucial thing might not be the polyA+ RNA isolation itself - which
seems to be pretty well established - but the workup of your plants.

For that, best ask an Arabidopsis or other plant expert,

have a look at [Only registered users see links. ] and
[Only registered users see links. ]
=UTF-8&hl=de&btnG=Google+Suche&meta=

There are lots of companies selling kits for your purpose, you might check
e.g. [Only registered users see links. ], [Only registered users see links. ], probably also
[Only registered users see links. ], Sigma and Qiagen.

All the best, Wo


At 19:42 24.08.2003 +0100, ip singh wrote:

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