I am trying to use semi-Q-PCR to study gene expression in different tissues. but I still can not figure out ,how to
use internal control (beta-acin) to verify the equal amounts of RNA BE USED from different
tissues? or, another word, how to normalize my cDNA?
I got two procedures from "biologicalprocedures", and read again and again,
still confused, how to normalize cDNA from different sample RNA?
If you have experience, please give me some advice, I am highly appreciated.
I am using the LightCycler for several years now. The way we normalise is by
using beta-2 microglobulin (according to the literature this is a better
I use the Ct crossing point of the two seperate reactions . Each reaction is
correlated to a standard and after doing this the two reactions of each
sample are correlated to eachother. This doesn't make sense reading it, but
I hope you will understand. If not let me know, I will send you an excell
template for your calculations.
<[Only registered users see links. ]> wrote:
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tissues. but I still can not figure out ,how to
USED from different
appreciated. [Only registered users see links. ]