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#1
08-07-2003, 07:37 PM
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 RACE cloning Hi, I have been trying to clone my gene of interest using "rapid amplification of cDNA ends (RACE)". I purchased the cDNA (double-stranded) from Clontech. So I did my first set of PCR using adaptor primer and my gene specific primer to perform 5' RACE or 3' RACE. But I got no bands. When I perform a second set of PCR (nested PCR), I got basically smears on the gel. I varied temperatures and cycling programs etc., but I still got smears instead of distinct bands. So I would like to ask if anyone has done similar things or if anyone has some suggestions. Any suggestions will be appreciated. Thanks. Emily  |
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