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detaching adherent cells

detaching adherent cells - Protocols and Methods Forum

detaching adherent cells - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-27-2003, 02:19 PM
Antonio Sarikas
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Default detaching adherent cells




Does anybody know an apporpriate way to detach cardiomyocytes form a
petri dish in order to collect for an Luciferase based atp assay?

Thanky a lot
Antonio



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Old 08-22-2003, 10:56 AM
Bob Hartley
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Default detaching adherent cells

Antonio Sarikas <[Only registered users see links. ]-erlangen.de> wrote in message news:<[Only registered users see links. ]-erlangen.de>...

Never done this with cardiomyocytes, but I would assume a standard
trypsin protocol.

EG wash with versene/edta soln then trypsin liftoff then serup or
peptide neutralisation of the trypsin.

The man that would know specifically for CM's is on holiday. If you
dont get any luck mail me and remind in 2 weeks


cheers
bob
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