| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
07-27-2003, 02:19 PM
| |||
| |||
 detaching adherent cells Does anybody know an apporpriate way to detach cardiomyocytes form a petri dish in order to collect for an Luciferase based atp assay? Thanky a lot Antonio  |
|
#2
08-22-2003, 10:56 AM
| |||
| |||
| detaching adherent cells Antonio Sarikas <[Only registered users see links. ]-erlangen.de> wrote in message news:<[Only registered users see links. ]-erlangen.de>... Never done this with cardiomyocytes, but I would assume a standard trypsin protocol. EG wash with versene/edta soln then trypsin liftoff then serup or peptide neutralisation of the trypsin. The man that would know specifically for CM's is on holiday. If you dont get any luck mail me and remind in 2 weeks cheers bob |
|
| Tags |
| adherent , cells , detaching |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| mouse hepatocytes isolation | amicia | Cell Biology and Cell Culture | 39 | 05-11-2012 11:52 AM |
| Characteristics of cancerous cells | aftabac | Cell Biology and Cell Culture | 2 | 08-26-2011 11:40 PM |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| How to isolate adult from circulation? | peterish | Stem Cell Forum | 0 | 05-19-2008 10:02 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
Linear Mode
