Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protocols and Methods Forum
Register Search Today's Posts Mark Forums Read

Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


keratinase

keratinase - Protocols and Methods Forum

keratinase - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-06-2003, 05:31 PM
peddu
Guest
 
Posts: n/a
Default keratinase



hi,
which chemical is more efficient in the strain
improvement(mutagenesis)of Bacillus producing keratinase.?
jeeva
Reply With Quote
  #2  
Old 07-07-2003, 11:40 AM
Wolfgang Schechinger
Guest
 
Posts: n/a
Default keratinase

Dear Jeeva,

that's difficlult to say. Best, you screen the literature (e.g. in
[Only registered users see links. ]) for 'directed evolution'. Look out for the reviews /
papers / articles by Willem P. Stemmer, Frances H. Arnold or Andreas
Pluckthun to get some ideas on current methods of mutagenesis and
screening. I am just afraid that there are as many methods as there are
papers (almost, of course). You will have to adapt them on your problem.

Alternatively, you simply could try a deliberated minimally dosed UV
irradiation of freshly seeded bacteria on agar plates and then screen the
colonies if you have an easy to perform assay (non-destructive on the plate
or in colony-lift style) for keratinase.

How do you plan to screen for positives and what kind of improvement do you
desire?

Wo

At 10:31 06.07.2003 -0700, peddu wrote:

---
Reply With Quote
Reply

Tags
keratinase


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 08:32 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11241 seconds with 15 queries