| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Protocols and Methods Forum Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
07-03-2003, 01:01 PM
| |||
| |||
 RNA isolation from frozen buffy coat Hi, could someone give me some suggestions how to increase the yield when isolating total RNA from frozen buffy coat using the Trizol LS method? Now I add Trizol LS to the buffy coat even before I start thawing it because I am afraid RNases will work in the buffy coat. When thawed the buffy coat/Trizol LS solution is difficult to pipette, very viscous. I will be thankful for any help. Anita  |
|
| Tags |
| buffy , coat , frozen , isolation , rna |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| RNA isolation from large frozen tissue | stefulj | RNA Techniques Forum | 5 | 07-06-2008 05:32 AM |
| frozen whole blood DNA isolation | lakshb | Basic Lab Protocols and Techniques | 1 | 07-27-2007 02:21 AM |
| Buffy Coat Cell Perparation | Fred | Protocols and Methods Forum | 1 | 09-20-2004 12:45 AM |
| Buffy Coat Cell Perparation | Fred | Protocols and Methods Forum | 0 | 08-25-2004 10:29 PM |
| Isolation of buffy coat (WBC) from whole blood (only 500 µl) after removing the plasma | Elisabet W | Protocols and Methods Forum | 0 | 03-02-2004 11:48 AM |
Linear Mode
