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RNA isolation from frozen buffy coat

RNA isolation from frozen buffy coat - Protocols and Methods Forum

RNA isolation from frozen buffy coat - Post Any Protocol, Method, Technique, Procedure or Tips / Troubleshooting for any Molecular Biology Technique.


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Old 07-03-2003, 01:01 PM
Anita
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Default RNA isolation from frozen buffy coat



Hi,

could someone give me some suggestions how to increase the yield when
isolating total RNA from frozen buffy coat using the Trizol LS method?
Now I add Trizol LS to the buffy coat even before I start thawing it
because I am afraid RNases will work in the buffy coat. When thawed
the buffy coat/Trizol LS solution is difficult to pipette, very
viscous. I will be thankful for any help.

Anita
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