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Octyl-D-Glucoside solutions

Octyl-D-Glucoside solutions - Protocols and Methods Forum

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  #1  
Old 07-03-2003, 01:18 AM
Andrew Perry
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Default Octyl-D-Glucoside solutions



Hi,

I have a collegue who is trying to make up Octyl-D-Glucoside in water at
20mM (or 40mM) which around the CMC. While this detergent is supposed to
be soluble to 20 %, his solutions appear immicible - he just gets phase
seperation or an opaque emulsion.

The stock he is using is 'Practical Grade' from ICN and is a yellow
liquid ... others have noted that Octyl-D-Glucoside comes as a
crystaline powder ...

Anyone have any experience with this (or similar) detergents ? How do
you get them to go into solution ?

Thanks,

Andrew

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  #2  
Old 07-03-2003, 01:38 AM
D.K.
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Default Octyl-D-Glucoside solutions

In article <be0048$muh$[Only registered users see links. ].unimelb.edu.au>, Andrew Perry <[Only registered users see links. ].Melbourne> wrote:

Both of these things tell me there is some sort of confusion and
he's got some other detergent.

DK
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  #3  
Old 07-03-2003, 02:45 AM
Andrew Perry
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Default Octyl-D-Glucoside solutions

The bottle says Octyl-D-Glucoside so I'd assume it is what we think it
is .... unless ICN has stuffed up and put something else in the bottle
?? It's also sometimes called n-OCTYL-D-GLUCOPYRANOSIDE.

Several papers refer to using Octyl-D-Glucoside at similar
concentrations, but purchased from Bachem or Anatrace. Maybe there is a
signifacant difference between 'Practical grade' and something else ?

Andrew

D.K. wrote:
<[Only registered users see links. ].Melbourne> wrote:

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  #4  
Old 07-07-2003, 03:07 PM
Dr Engelbert Buxbaum
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Default Octyl-D-Glucoside solutions

Andrew Perry wrote:



Pure Octyl-glucoside is a white powder, and highly water soluble.
Companies like Anatrace sell detergents wich are highly purified and
certified for use in membrane protein solubilisation.

You can also buy technical grade detergents, which are used to make
cleaning fluids, washing powder and similar products. These are neither
purified nor characterised to the same standards, and a method that may
work well with one batch may completely fail with another.


As to your friend he probably got something different, IIRC OG in
aqueous solutions may slowly hydrolyse, such solutions should be freshly
prepared.
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